My brothers cried Essay Example for Free

My brothers cried Essay Originally is a poem set upon the main themes of growing up. By looking at the poem in depth, I have come to a conclusion that Carol Ann Duffy is the persona who is writing with a childs perspective as when she was a child. In verse one, The poet creates a sense of noise and chaos by saying how each member of her family are making noise and moving. Mother singing My brothers cried both of these quotes contribute to the feeling of noise and chaos. Two metaphors, which are found in the poem by Carol Ann Duffy, could be fell through the fields miles rushed back. They show that they were in the countryside. It also shows that they are using some form of transport, which may be train or car. I assumed by reading the poem that the reader was a young girl, and when she is sitting down where on the journey, it feels like you are stationary so it feels as if everything is rushing towards yourself. This demonstrates the Childs prospective. On the last line of the first verse, the metaphor shows that the young girl is looking at one of her toys, which maybe a bear with button eyes, and as she has grown up a fraction more than she was, she may now understand that toys do not have feelings, and they are only buttons as the eyes so they cannot really see things. She makes us feel like a child is commenting here because when youre at such a young age, you think that toys really do live on their own world and they have the same senses as us. I. e. touch, taste, feel, hear, pain etc. I think that the whole poem focuses mainly on sudden interruptions because from each paragraph is a different age section of her life. You can also see by the poem that as each paragraph develops, the way it is written and the style of wording develops, as if the child is becoming increasingly mature and adult-like. The Childs vulnerability is shown in verse two by using such words as big boys eating worms and shouting words you didnt understand perhaps this is hinting that maybe the big boys who were eating worms were swearing or saying words which she was too young to have come across. The last word in verse two is italicised because the narrator (who is a daughter) is speaking and it also shows that she is missing where she used to live as a child. In verse three, the child is less vulnerable now she has settled in because she is now used to everything. But then you forget this shows that now the narrator has lived for quite a few years, she tends to forget her past easier. The narrator now speaks in a comforted tone and gives away the idea of being a lot more worried about life in general as compared to when she was younger and slightly unsure about moving. Now that she has fitted in well, she now understands how it feels like to be part of their community. The change is compared to the shedding of a snakes skin. They are compared with each other because it is in conjunction with her voice changing i. e. maybe she has changed language or they use a different accent. Or simply her voice could have just changed with age. Now that she is used to the changes, she feels as if she has completely lost her old voice I lost a river, culture, speech, sense of first space This could potentially be a good or bad thing but we assume it is for good because she prefers being accepted and fitting in when she didnt think she would ever. The person created in the poem, we could comfortably say that it is a girl, even though no evidence backs up this statement. I assume by reading between the lines that she is around the age of 8-12 because on the journey, she sees thing not as clearly as adults do. My voice in the classroom sounding just like the rest This also shows that she is a school pupil so she must be fairly young. The setting in the poem is created by the feelings shown by the young girl. Fell through the fields This shows that they used to live in the country side, but they moved to a rural area where flats and estates can be found Pebble-dashed estates So maybe the girl is unsure of the area when she realises that where she is moving to is a lot more busy and lively. The use of 3 verses is a very good idea for several reasons. For one, it defines the maturing of her age, which is put across using methods such as different tones of voice, and more complex metaphors and sentences. The whole poem could be mapped out as journey. The first verse demonstrates the beginning of her life and when the family move. Second verse indicating the actual journey, and the becoming older stage, whilst trying to get used to moving. With the last verse when she has settled in her new area, and it has a quote on the last line which demonstrates her socialising with her friends, and seems to have fitted in really well.

Yeast Two-Hybrid Essay -- Biology, DNA

The initial transformation task required two yeast strains (both as Mav203; Leu–, Trp–, His–), with one containing the pDBLeuDa4 bait plasmid, and the other with just the pDBLeu plasmid. The two strains were each transformed with a pPC86DMID1(Y) prey plasmid, where (Y) referred to a full-length positive control (MID1) , a DBB strain (MID1DBB), and a DCC strain (MID1DCC). The transformation process with the two strains and three prey plasmid DNAs resulted in 6 (2x3) transformation mixtures (Table 1). The transformation mixtures were then spread-plated at 1x and 1/10x dilutions using half of each plate on an SC (synthetic complete) yeast growth medium minus selected amino acid(s); one plate without Leucine (SC-Leu), and two plates without Leucine and Tryptophan (SC-Leu-Trp). Plates were then incubated for 4 days at 30 ° C, however due to failed growth in the transformation plates for pPC86DMID1 (both yeast strains), and an additional set of results had to be obtained. The spread-plated results for each strain, transformation, result source, and their associated control cases can be seen in Table 2, with a â€Å"Yes† growth observation referring to confluent growth and â€Å"No† for non-confluent growth. Following the incubation period, each of the 6 transformation plates were sampled and underwent 4x 10-fold serial dilutions, which were then spot-tested to either an SC-Leu-Trp, or SC-Leu-Trp-His (minus Histidine) medium. Each strain-transformation occupied half of each plate, with a spot per dilution level (Figure 1). An additional set of spot-tests were also performed to test for auto-activation of the a4 bait protein, using a strain transformed with an unmodified pPC86 plasmid (MaV203+pDBLeuDa4+pPC86). Following another round of in... ...the DCC variant (for all spot dilution levels). The lack of growth for the DBB variant suggested the B-box region was a requirement for interaction/binding with the a4 protein, while the coiled-coil domain didn’t appear to be a requirement. Its removal however did result in noticeably decreased growth rates when compared to the full-length control test, suggesting its removed resulted in some level of decreased interaction with a4, whether it usually stabilised the binding process, or is removal disrupted the overall form and binding site is unknown, and would require additional testing. However given the external result source, it could have been that group’s sampling and dilution methods had resulted in decreased cell viability, though a simple retest could rule this out, and would be a good starting point to determine any interactions with the coiled-coil domain.

Assignment- Final Paper Essay

These last five weeks in EXP 105 has really helped me learn a lot about learning in general, and also it has helped me to understand myself as a learner. If I had to explain the concept of learning to someone who has not yet taken this class, I would tell them that it is simply knowing who you are as learner and knowing what are your intentions are when it comes to learning. Intentions alone play a big part in learning because that is the ultimate goal; becoming an intentional learner. An intentional learner knows how to approach learning by developing skills to read, write, and think critically. I always felt that learning itself was a full time job, but this class changed that view by teaching me how to learn, and also what my weak and strong points were. This helps you to manage time, and learning no longer feels like it takes forever. In this class we were given a learning connections inventory (LCI). It was a short survey to help us identify our learning patterns. My LCI scores were as follows: Sequence 27, Precision 28. Technical Reasoning 20, and Confluence 17. Sequence and precision are my two use first learning patterns. I use these patterns on the regular. I am a very organized person and I like to make sure that I have all the information that is necessary before I perform a task. I use technical reasoning as needed, and then I avoid Confluence. Confluence requires a lot of creativity and that is the area I lack in the most so I try to stay away from it as much as I can. No matter how we use our learning patterns though, it always comes a time where we must use them all whether we realize it or not. Sometimes it is necessary to combine all of the learning patterns to get a full understanding of what is happening, or supposed to happen. The benefits of knowing your unique learning patterns are for one you already know how you should approach different tasks to get them done. It takes the guesswork out and saves you time to go back and finish something you did not  understand later. While learning patterns are strong tools to have in your tool box by themselves, sometimes it is necessary to have strategies to go along with them. When it comes to sequence I do not always think that a strategy is necessary because it is always important for things to be organized for them to look neat and flow smoothly when it comes too writing at least, and that is how I tried to think about every learning pattern and assignment in this class. My strategy for mu use first precision is to keep it at a minimum without being boring, less really is more sometimes. Technical reasoning will remain as it is since it is use as needed. I definitely know that my strategy for confluence was to intensify it a little more so that I can try to warm up to it and try to incorporate it into more of my assignments. The way that I took advantage of my strengths in this course was actually using them when I was completing assignments. The biggest challenge of my learning patterns was figuring out when and how to use them. It gradually got better once we discussed FITing and decoding assignments. It did not take long for me to grasp those concepts and after that everything became clear and it all made sense on hoe it all fit together. That solved the problem of the learning patterns being hard to understand when it came to using them to complete assignments in the course. I didn’t get to use the FITing concept until the end, but I wish I would have known about it earlier. I definitely plan to use it in my future courses because it makes everything easier to understand and I enjoyed doing it. I didn’t think of it as extra unnecessary work. It was more like a map to me to tell me what I should do. In the future I will also use more of the library and student resources to help me with FITing. The thing that I enjoyed the most about this class w being given the opportunity to learn about myself and who I am as a learner. I definitely feel different about learning and I plan to use everything I learned in this course in future courses. My favorite assignments to do in this class were the LCI and then the FITing and decoding assignment. To make the class a little better and more fun I think at least another variation of the LCI should be included. I had taken online courses before so all of my expectations were met. I enjoyed this class overall.

The Empathy Of Immersion Through Video Games - 3308 Words

Glenn Atmadji 1 The Empathy of Immersion in Sex and Sexuality in Video Games The way one presents oneself is usually an ample enough reason to be judged positively or negatively. This factor is usually amplified by the surrounding and setting set for the subject, and it usually determines whether the subject’s self-presentation is fitting or being completely inappropriate to his or her surroundings. In video games, the issue of women being misrepresented by her role as a sex object and over-sexualized aesthetic design had always been an unsolved floating problem. While this dilemma continue to exist as a negativity in the modern video games is often overlooked as a meaningless eye candy for the male gaze, sometimes the subject of sex and sexuality in video game exists for a reason much deeper than simply objectifying women for the sake of sales or male gaze. Although not always, sex and sexuality exists to add a certain of immersion of realism to the game as the subject is a natural part of our biology as human beings. One of the unfortunate victim from the finger pointing of female objectification case is the otherwise harmless video game is the Gothic (Piranha Bytes, 2001) series, a single-player action role-playing video game developed by German company Piranha Bytes for the PC. Gothic was released first in Germany back on March at 2001 and the game was rated positively by critics and fans alike. For a game with a few English translation issue and plenty ofShow MoreRelatedThe Relationship Between Democracy And Civility1900 Words   |  8 Pagesand video games. â€Å"Although no individual risk factor may be sufficient to cause aggressive or violent behavior on its own, each factor increases the likelihood of aggressive thoughts, behaviors, feelings and the decline of pro-social behavior† (Anderson, 2001). 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